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Anti-NFIA Antibody Picoband®, APC Conjugate

Boster Bio Anti-NFIA Antibody Picoband® catalog # A03531. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nuclear factor 1 A-type is a protein that in humans is encoded by the NFIA gene. This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name

NFIA

Gene ID

4774

UniProt

Q12857

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

E.coli-derived human NFIA recombinant protein (Position: V172-Q233). Human NFIA shares 100% amino acid (aa) sequence identity with rat NFIA.

Clonality

Polyclonal

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Campbell, C. G. N., Wang, H., Hunter, G. W. Interstitial microdeletion of chromosome 1p in two siblings. Am. J. Med. Genet. 111: 289-294, 2002. 2. das Neves, L., Duchala, C. S., Godinho, F., Haxhiu, M. A., Colmenares, C., Macklin, W. B., Campbell, C. E., Butz, K. G., Gronostajski, R. M. Disruption of the murine nuclear factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus, and agenesis of the corpus callosum. Proc. Nat. Acad. Sci. 96: 11946-11951, 1999. Note: Erratum: Proc. Nat. Acad. Sci. 98: 4276 only, 2001. 3. Deneen, B., Ho, R., Lukaszewicz, A., Hochstim, C. J., Gronostajski, R. M., Anderson, D. J. The transcription factor NFIA controls the onset of gliogenesis in the developing spinal cord. Neuron 52: 953-968, 2006.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

56 kDa

Observed Molecular Weight

70 kDa

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

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