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Anti-HIBADH Antibody Picoband®, APC Conjugate

Boster Bio Anti-HIBADH Antibody Picoband® catalog # A08760-3. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

In enzymology, a 3-hydroxyisobutyrate dehydrogenase also known as β-hydroxyisobutyrate dehydrogenase or 3-hydroxyisobutyrate dehydrogenase, mitochondrial (HIBADH) is an enzyme that in humans is encoded by the HIBADH gene. This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.

Gene Name

HIBADH

Gene ID

11112

UniProt

P31937

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

E.coli-derived human HIBADH recombinant protein (Position: R34-E332). Human HIBADH shares 93.3% and 92.6% amino acid (aa) sequence identity with mouse and rat HIBADH, respectively.

Clonality

Polyclonal

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Rougraff, P. M., Zhang, B., Kuntz, M. J., Harris, R. A., Crabb, D. W. Cloning and sequence analysis of a cDNA for 3-hydroxyisobutyrate dehydrogenase: evidence for its evolutionary relationship to other pyridine nucleotide-dependent dehydrogenases. J. Biol. Chem. 264: 5899-5903, 1989.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

32 kDa

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

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