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Anti-GLT25D1/COLGALT1 Antibody Picoband®, APC Conjugate

Selected Variant

A12583-1-APC

Size: 100 µg/Vial

Boster Bio Anti-GLT25D1/COLGALT1 Antibody Picoband® catalog # A12583-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum.

Gene Name

COLGALT1

Gene ID

79709

UniProt

Q8NBJ5

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

E.coli-derived human COLGALT1 recombinant protein (Position: A34-D454). Human GLT25D1/COLGALT1 shares 93.6% amino acid (aa) sequence identity with mouse COLGALT1.

Clonality

Polyclonal

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Baumann, S., Hennet, T. Collagen accumulation in osteosarcoma cells lacking GLT25D1 collagen galactosyltransferase. J. Biol. Chem. 291: 18514-18524, 2016. 2. Hartz, P. A. Personal Communication. Baltimore, Md. June 9, 2017. 3. Liefhebber, J. M. P., Punt, S., Spaan, W. J. M., van Leeuwen, H. C. The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein. BMC Cell Biol. 11: 33, 2010. Note: Electronic Article.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

85 kDa

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

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