Anti-MIOX Antibody Picoband®, APC Conjugate

Boster Bio Anti-MIOX Antibody Picoband® catalog # A06957. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Myo-inositol oxygenase (MIOX), also known as ALDRL6, is a renal-specific member of the Aldo-keto reductase family. It catalyzes the first committed step in the Myo-inositol metabolism pathway and is widely distributed in mammalian tissues. Human Myo-inositol oxygenase shares 91% and 96% sequence homology with mouse and pig Myo-inositol oxygenase homologs, respectively. Myo-inositol oxygenase is responsible for the oxidative cleavage of Myo-inositol (MI) and its epimer D-chiro inositol (DCI) to D-glucuronate. The dioxygen-dependent cleavage of the C1-C6 bond in Myo-inositol is accomplished through the utilization of the FeII/FeIII binuclear iron center of MIOX. Myo-inositol oxygenase has also been implicated in complications of diabetes, including diabetic nephropathy.

Gene Name

MIOX

Gene ID

55586

UniProt

Q9UGB7

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

E.coli-derived human MIOX recombinant protein (Position: M1-K266). Human MIOX shares 89.8% and 90.5% amino acid (aa) sequence identity with mouse and rat MIOX, respectively.

Clonality

Polyclonal

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Arner, R. J., Prabhu, K. S., Thompson, J. T., Hildenbrandt, G. R., Liken, A. D., Reddy, C. C. Myo-inositol oxygenase: molecular cloning and expression of a unique enzyme that oxidizes myo-inositol and D-chiro-inositol. Biochem. J. 360: 313-320, 2001. 2. Gross, M. B. Personal Communication. Baltimore, Md. 3/31/2016. 3. Yang, Q., Dixit, J. B., Wada, J., Tian, Y., Wallner, E. I., Srivastva, S. K., Kanwar, Y. S. Identification of a renal specific oxido-reductase in newborn diabetic mice. Proc. Nat. Acad. Sci. 97: 9896-9901, 2000.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

36 kDa

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse Immunohistochemistry, 2-5 μg/ml, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -