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Anti-PHPT1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-PHPT1 Antibody Picoband® catalog # A06841. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

14 kDa phosphohistidine phosphatase is an enzyme that in humans is encoded by the PHPT1 gene. This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants.

Gene Name

PHPT1

Gene ID

29085

UniProt

Q9NRX4

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

E.coli-derived human PHPT1 recombinant protein (Position: M1-Y125). Human PHPT1 shares 84.4% amino acid (aa) sequence identity with mouse PHPT1.

Clonality

Polyclonal

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ek, P., Pettersson, G., Ek, B., Gong, F., Li, J.-P., Zetterqvist, O. Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase. Europ. J. Biochem. 269: 5016-5023, 2002.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

14 kDa

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry, 2-5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

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