Anti-NUCKS1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-NUCKS1 Antibody Picoband® catalog # A04997-3. Tested in IF, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nuclear ubiquitous casein and cyclin-dependent kinases substrate is a protein that in humans is encoded by the NUCKS1 gene. This gene encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle.

Synonyms

Fms-related tyrosine kinase 3 ligand ;Flt3lg ;

Gene Name

fms-related tyrosine kinase 3 ligand

Gene ID

64710

UniProt

Q9H1E3

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human NUCKS1, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Applications

IF,IHC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Grundt, K., Haga, I. V., Aleporou-Marinou, V., Drosos, Y., Wanvik, B., Ostvold, A. C. Characterisation of the NUCKS gene on human chromosome 1q32.1 and the presence of a homologous gene in different species. Biochem. Biophys. Res. Commun. 323: 796-801, 2004. 2. Ostvold, A. C., Norum, J. H., Mathiesen, S., Wanvik, B., Sefland, I., Grundt, K. Molecular cloning of a mammalian nuclear phosphoprotein NUCKS, which serves as a substrate for Cdk1 in vivo. Europ. J. Biochem. 268: 2430-2440, 2001.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

25847 MW

Observed Molecular Weight

20 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/ml, Human, Mouse Immunofluorescence, 5 μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human