Anti-MMP19 Antibody Picoband®, APC Conjugate

Boster Bio Anti-MMP19 Antibody Picoband® catalog # A05171-3. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Matrix metalloproteinase-19 (MMP-19) also known as matrix metalloproteinase RASI is an enzyme that in humans is encoded by the MMP19 gene. This gene encodes a member of a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded protein is secreted as an inactive proprotein, which is activated upon cleavage by extracellular proteases. Alternative splicing results in multiple transcript variants for this gene.

Synonyms

Protein Dok-7; Downstream of tyrosine kinase 7; DOK7; C4orf25;

Gene Name

docking protein 7

Gene ID

4327

UniProt

Q99542

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MMP19 recombinant protein (Position: L120-D437).

Clonality

Polyclonal

Tissue Specificity

Preferentially expressed in skeletal muscle and heart. Present in thigh muscle, diaphragm and heart but not in the liver or spleen .

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Cossins, J., Dudgeon, T. J., Catlin, G., Gearing, A J. H., Clements, J. M. Identification of MMP-18, a putative novel human matrix metalloproteinase. Biochem. Biophys. Res. Commun. 228: 494-498, 1996. 2. Hazlewood, R. J., Roos, B. R., Solivan-Timpe, F., Honkanen, R. A., Jampol, L. M., Gieser, S. C., Meyer, K. J., Mullins, R. F., Kuehn, M. H., Scheetz, T. E., Kwon, Y. H., Alward, W. L. M., Stone, E. M., Fingert, J. H. Heterozygous triplication of upstream regulatory sequences leads to dysregulation of matrix metalloproteinase 19 in patients with cavitary optic disc anomaly. Hum. Mutat. 36: 369-378, 2015. 3. Moore, M., Salles, D., Jampol, L. M. Progressive optic nerve cupping and neural rim decrease in a patient with bilateral autosomal dominant optic nerve colobomas. Am. J. Ophthal. 129: 517-520, 2000.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

67074 MW

Observed Molecular Weight

54 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human Immunohistochemistry, 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -