Anti-PPP1R15B Antibody Picoband®, APC Conjugate

Boster Bio Anti-PPP1R15B Antibody Picoband® catalog # A10225-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

PPP1R15B(Protein phosphatase 1, regulatory subunit 15b), also called CREP, promotes dephosphorylation of the transcription initiation factor EIF2-alpha through recruitment of protein phosphatase-1(PP1) catalytic subunits. The PPP1R15B gene is mapped to chromosome 1q32.1 based on an alignment of thePPP1R15B sequence by Hartz(2010). Harding et al.(2009) obtained Ppp1r15b -/- mice at a mendelian ratio. However, Ppp1r15b -/- newborns were half the size of their wildtype littermates, were notably pale, and failed to nurse, and none survived the first day of postnatal life. Ppp1r15b -/- embryos that were also homozygous for an Eif2-alpha mutation that prevented Eif2-alpha phosphorylation were normalized, including elevated birth size and restored red blood cell count, compared with Ppp1r15b -/- embryos with wildtype Eif2-alpha.

Synonyms

Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3;

Gene Name

Protein NDRG3

Gene ID

84919

UniProt

Q5SWA1

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PPP1R15B recombinant protein (Position: Q211-D657).

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Highly expressed in brain. .

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Harding, H. P., Zhang, Y., Scheuner, D., Chen, J.-J., Kaufman, R. J., Ron, D. Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2-alpha) dephosphorylation in mammalian development. Proc. Nat. Acad. Sci. 106: 1832-1837, 2009.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

41409 MW

Observed Molecular Weight

110 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -