Anti-PPM1N Antibody Picoband®, APC Conjugate

Boster Bio Anti-PPM1N Antibody Picoband® catalog # A16917-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Predicted to enable metal ion binding activity and protein serine/threonine phosphatase activity. Predicted to be involved in negative regulation of I-kappaB kinase/NF-kappaB signaling and positive regulation of canonical Wnt signaling pathway. Predicted to be active in cytosol and nucleus.

Synonyms

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

Gene Name

kelch repeat and BTB domain containing 2

Gene ID

147699

UniProt

Q8N819

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PPM1N recombinant protein (Position: R36-A430).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Zhou, H. , Xu, M. , Huang, Q. , Gates, A. T. , Zhang, X. D. , & Castle, J. C. , et al. (2008). Genome-scale rnai screen for host factors required for hiv replication. Cell host & microbe, 4(5), 495-504. 2. Shi, P. , Guo, Y. , Su, Y. , Zhu, M. , & Huang, J. . (2020). Sumoylation of ddx39a alters binding and export of antiviral transcripts to control innate immunity. The Journal of Immunology, 205(1), ji2000053. 3. Fasci, D. , Ingen, H. V. , Scheltema, R. A. , & Heck, A. J. R. . (2018). Histone interaction landscapes visualized by crosslinking mass spectrometry in intact cell nuclei. Molecular & Cellular Proteomics Mcp, 17(10), mcp.RA118.000924.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

24145 MW

Observed Molecular Weight

45 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -