Anti-Renin/REN Antibody Picoband®, APC Conjugate

Boster Bio Anti-Renin/REN Antibody Picoband® catalog # A03689-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis.

Synonyms

p-selectin glycoprotein ligand; Selplg; Psgl1

Gene Name

selectin P ligand

Gene ID

5972

UniProt

P00797

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Renin/REN recombinant protein (Position: N80-D370).

Clonality

Polyclonal

Tissue Specificity

Highly expressed in blood, bone marrow, brain, adipose tissue, spleen, and thymus. Also expressed in heart, kidney, liver, muscle, ovary, and stomach.

Applications

WB,IHC,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

A SLe (x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Abel, K. J., Gross, K. W. Close physical linkage of the murine Ren-1 and Ren-2 loci. Nucleic Acids Res. 16: 2111-2126, 1988. 2. Caron, K. M. I., James, L. R., Kim, H.-S., Knowles, J., Uhlir, R., Mao, L., Hagaman, J. R., Cascio, W., Rockman, H., Smithies, O. Cardiac hypertrophy and sudden death in mice with a genetically clamped renin transgene. Proc. Nat. Acad. Sci. 101: 3106-3111, 2004. 3. Chirgwin, J. M., Schaefer, I. M., Diaz, J. A., Lalley, P. A. Mouse kidney renin gene is on chromosome one. Somat. Cell Molec. Genet. 10: 633-637, 1984.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.