Anti-RUFY4 Antibody Picoband®, APC Conjugate

Boster Bio Anti-RUFY4 Antibody Picoband® catalog # A16707-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Enables phosphatidylinositol-3-phosphate binding activity. Involved in autophagosome assembly; cellular response to interleukin-4; and positive regulation of macroautophagy. Located in autophagosome.

Synonyms

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

Gene Name

kelch repeat and BTB domain containing 2

Gene ID

285180

UniProt

Q6ZNE9

Host

Rabbit

Reactivity

Human,Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RUFY4 recombinant protein (Position: M1-K553).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Terawaki, S. , Camosseto, V. , Prete, F. , Wenger, T. , Papadopoulos, A. , & Rondeau, C. , et al. (2015). Run and fyve domain-containing protein 4 enhances autophagy and lysosome tethering in response to interleukin-4. The Journal of Cell Biology, 210(7), 1133-1152. 2. Chen, S. , Robert, F. , Lambertus, K. , Yuan, L. , Wang, J. , & Kathryn, R. , et al. (2018). An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders. Nature Genetics. 3. Haenig, C. , Atias, N. , Taylor, A. K. , Mazza, A. , & Wanker, E. E. . (2020). Interactome mapping provides a network of neurodegenerative disease proteins and uncovers widespread protein aggregation in affected brains. Cell Reports, 32(7), 108050.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

24145 MW

Observed Molecular Weight

64 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Monkey Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -