Anti-SPHK1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-SPHK1 Antibody Picoband® catalog # A01390-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

Synonyms

Hexokinase-2;2.7.1.1;Hexokinase type II;HK II;Muscle form hexokinase;HK2;

Gene Name

Hexokinase-2

Gene ID

8877

UniProt

Q9NYA1

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SPHK1 recombinant protein (Position: E55-N357).

Clonality

Polyclonal

Tissue Specificity

Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Kohama, T., Olivera, A., Edsall, L., Nagiec, M. M., Dickson, R., Spiegel, S. Molecular cloning and functional characterization of murine sphingosine kinase. J. Biol. Chem. 273: 23722-23728, 1998. 2. Melendez, A. J., Carlos-Dias, E., Gosink, M., Allen, J. M., Takacs, L. Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution. Gene 251: 19-26, 2000. 3. Pitson, S. M., D'Andrea, R. J., Vandeleur, L., Moretti, P. A. B., Xia, P., Gamble, J. R., Vadas, M. A., Wattenberg, B. W. Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem. J. 350: 429-441, 2000.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

102380 MW

Observed Molecular Weight

52 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -

Subcellular Location

Mitochondrion outer membrane . Its hydrophobic N-terminal sequence may be involved in membrane binding. .

Protein Name

Hexokinase-2

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

GTR15157480 1 Vial

Lentiviral mouse Plvap shRNA (UAS) - Lentiviral mouse Plvap shRNA (UAS, iRFP) plasmid

Ask

View Details

131106-ML650 100 µL

PDLIM5 (PDZ and LIM Domain Protein 5, Enigma Homolog, Enigma-like PDZ and LIM Domains Protein, ENH, L9) (MaxLight 650)

Ask

View Details

A72467-50UG 50 µg

AXIN2 Antibody, FITC conjugated

Ask

View Details

MBS2034060-01 0.1 mL

Polyclonal Antibody to Protein Tyrosine Phosphatase, Non Receptor Type 13 (PTPN13)

Ask

View Details

MBS2034060-02 0.2 mL

Polyclonal Antibody to Protein Tyrosine Phosphatase, Non Receptor Type 13 (PTPN13)

Ask

View Details

MBS2034060-03 0.5 mL

Polyclonal Antibody to Protein Tyrosine Phosphatase, Non Receptor Type 13 (PTPN13)

Ask

View Details