Anti-Claudin 1/CLDN1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-Claudin 1/CLDN1 Antibody Picoband® catalog # A01585-3. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Synonyms

Growth/differentiation factor 15;GDF-15;Macrophage inhibitory cytokine 1;MIC-1;NSAID-activated gene 1 protein;NAG-1;NSAID-regulated gene 1 protein;NRG-1;Placental TGF-beta;Placental bone morphogenetic protein;Prostate differentiation factor;GDF15;MIC1, PDF, PLAB, PTGFB;

Gene Name

Growth/differentiation factor 15

Gene ID

9076

UniProt

O95832

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Claudin 1/CLDN1 recombinant protein (Position: F96-V211).

Clonality

Polyclonal

Tissue Specificity

Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

Applications

WB,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Baala, L., Hadj-Rabia, S., Hamel-Teillac, D., Hadchouel, M., Prost, C., Leal, S. M., Jacquemin, E., Sefiani, A., de Prost, Y., Courtois, G., Munnich, A., Lyonnet, S., Vabres, P. Homozygosity mapping of a locus for a novel syndromic ichthyosis to chromosome 3q27-q28. J. Invest. Derm. 119: 70-76, 2002. 2. Coyne, C. B., Gambling, T. M., Boucher, R. C., Carson, J. L., Johnson, L. G. Role of claudin interactions in airway tight junctional permeability. Am. J. Physiol. Lung Cell. Molec. Physiol. 285: L1166-L1178, 2003. 3. Dhawan, P., Singh, A. B., Deane, N. G., No, Y., Shiou, S.-R., Schmidt, C., Neff, J., Washington, M. K., Beauchamp, R. D. Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer. J. Clin. Invest. 115: 1765-1776, 2005.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.