Anti-SGTA Antibody Picoband®, APC Conjugate

Boster Bio Anti-SGTA Antibody Picoband® catalog # A03818-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Small glutamine-rich tetratricopeptide repeat-containing protein alpha is a protein that in humans is encoded by the SGTA gene. This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.

Synonyms

SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2; UBLE1B; HRIHFB2115

Gene Name

ubiquitin like modifier activating enzyme 2

Gene ID

6449

UniProt

O43765

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SGTA recombinant protein (Position: M1-Q282).

Clonality

Polyclonal

Tissue Specificity

Duodenum, heart, lung and liver, but not thymus.

Applications

ELISA,IF,ICC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Kordes, E., Savelyeva, L., Schwab, M., Rommelaere, J., Jauniaux, J.-C., Cziepluch, C. Isolation and characterization of human SGT and identification of homologues in Saccharomyces cerevisiae and Caenorhabditis elegans. Genomics 52: 90-94, 1998. 2. Shao, S., Rodrigo-Brenni, M. C., Kivlen, M. H., Hegde, R. S. Mechanistic basis for a molecular triage reaction. Science 355: 298-302, 2017. 3. Tobaben, S., Thakur, P., Fernandez-Chacon, R., Sudhof, T. C., Rettig, J., Stahl, B. A trimeric protein complex functions as a synaptic chaperone machine. Neuron 31: 987-999, 2001.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64133 MW

Observed Molecular Weight

37 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -