Anti-ACAD9 Antibody Picoband®, APC Conjugate

Boster Bio Anti-ACAD9 Antibody Picoband® catalog # A05223-3. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Acyl-CoA dehydrogenase family member 9, mitochondrial is an enzyme that in humans is encoded by the ACAD9 gene. This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.

Synonyms

Histone deacetylase 10; HD10; HDAC10

Gene Name

histone deacetylase 10

Gene ID

28976

UniProt

Q9H845

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ACAD9 recombinant protein (Position: K92-C621).

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. High expression in liver, spleen, pancreas and kidney.

Applications

WB,IHC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Dewulf, J. P., Barrea, C., Vincent, M.-F., De Laet, C., Van Coster, R., Seneca, S., Marie, S., Nassogne, M.-C. Evidence of a wide spectrum of cardiac involvement due to ACAD9 mutations: report on nine patients. Molec. Genet. Metab. 118: 185-189, 2016. 2. Ensenauer, R., He, M., Willard, J.-M., Goetzman, E. S., Corydon, T. J., Vandahl, B. B., Mohsen, A.-W., Isaya, G., Vockley, J. Human acyl-CoA dehydrogenase-9 plays a novel role in the mitochondrial beta-oxidation of unsaturated fatty acids. J. Biol. Chem. 280: 32309-32316, 2005. 3. Haack, T. B., Danhauser, K., Haberberger, B., Hoser, J., Strecker, V., Boehm, D., Uziel, G., Lamantea, E., Invernizzi, F., Poulton, J., Rolinski, B., Iuso, A., Biskup, S., Schmidt, T., Mewes, H.-W., Wittig, I., Meitinger, T., Zeviani, M., Prokisch, H. Exome sequencing identifies ACAD9 mutations as a cause of complex I deficiency. Nature Genet. 42: 1131-1134, 2010.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

69 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -