Anti-TSPY1/2/3/4 Antibody Picoband®, APC Conjugate

Boster Bio Anti-TSPY1/2/3/4 Antibody Picoband® catalog # A04215-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Testis-specific Y-encoded protein 1/2/3/4 is a protein that in humans is encoded by the TSPY1/2/3/4 gene. The protein encoded by this gene is found only in testicular tissue and may be involved in spermatogenesis. Many functional paralogs and pseudogenes of this gene are present in a cluster in humans, but only a single, nonfunctional orthologous gene is found in mouse. Alternative splicing results in multiple transcript variants.

Synonyms

Transforming acidic coiled-coil-containing protein 1;Gastric cancer antigen Ga55;Taxin-1;TACC1;KIAA1103;

Gene Name

Transforming acidic coiled-coil-containing protein 1

Gene ID

7258/64591/728137/728395

UniProt

Q01534/A6NKD2/P0CV98/P0CV99

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TSPY1/2/3/4 recombinant protein (Position: R16-R247).

Clonality

Polyclonal

Tissue Specificity

Isoform 1, isoform 3 and isoform 5 are ubiquitous. Isoform 2 is strongly expressed in the brain, weakly detectable in lung and colon, and overexpressed in gastric cancer. Isoform 4 is not detected in normal tissues, but strong expression was found in gastric cancer tissues. Down-regulated in a subset of cases of breast cancer. .

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Likely involved in the processes that promote cell division prior to the formation of differentiated tissues.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Arnemann, J., Epplen, J. T., Cooke, H. J., Sauermann, U., Engel, W., Schmidtke, J. A human Y-chromosomal DNA sequence expressed in testicular tissue. Nucleic Acids Res. 15: 8713-8724, 1987. 2. Arnemann, J., Jakubiczka, S., Thuring, S., Schmidtke, J. Cloning and sequence analysis of a human Y-chromosome-derived, testicular cDNA, TSPY. Genomics 11: 108-114, 1991. 3. Guttenbach, M., Muller, U., Schmid, M. A human moderately repeated Y-specific DNA sequence is evolutionarily conserved in the Y chromosome of the great apes. Genomics 13: 363-367, 1992.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

87794 MW

Observed Molecular Weight

39 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -

Subcellular Location

Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Midbody . Nucleus during interphase. Weakly concentrated at centrosomes during mitosis and colocalizes with AURKC at the midbody during cytokinesis. .