Anti-UBA5 Antibody Picoband®, APC Conjugate

Boster Bio Anti-UBA5 Antibody Picoband® catalog # A06385-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Ubiquitin-like modifier-activating enzyme 5 is a protein that in humans is encoded by the UBA5 gene. This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1.

Synonyms

Rho-related GTP-binding protein Rho6;Rho family GTPase 1;Rnd1;RND1;RHO6;

Gene Name

Rho-related GTP-binding protein Rho6

Gene ID

79876

UniProt

Q9GZZ9

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human UBA5 recombinant protein (Position: R37-D389).

Clonality

Polyclonal

Tissue Specificity

Mostly expressed in brain and liver.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Colin, E., Daniel, J., Ziegler, A., Wakim, J., Scrivo, A., Haack, T. B., Khiati, S., Denomme, A.-S., Amati-Bonneau, P., Charif, M., Procaccio, V., Reynier, P., and 19 others. Biallelic variants in UBA5 reveal that disruption of the UFM1 cascade can result in early-onset encephalopathy. Am. J. Hum. Genet. 99: 695-703, 2016. 2. Dou, T., Gu, S., Liu, J., Chen, F., Zeng, L., Guo, L., Xie, Y., Mao, Y. Isolation and characterization of ubiquitin-activating enzyme E1-domain containing 1, UBE1DC1. Molec. Biol. Rep. 32: 265-271, 2005. 3. Duan, R., Shi, Y., Yu, L., Zhang, G., Li, J., Lin, Y., Guo, J., Wang, J., Shen, L., Jiang, H., Wang, G., Tang, B. UBA5 mutations cause a new form of autosomal recessive cerebellar ataxia. PLoS One 11: e0149039, 2016. Note: Electronic Article.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

26056 MW

Observed Molecular Weight

50 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -