Anti-VPS53 Antibody Picoband®, APC Conjugate

Boster Bio Anti-VPS53 Antibody Picoband® catalog # A06836-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human; Mouse; Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Vacuolar protein sorting 53 homolog (S. cerevisiae) is a protein that in humans is encoded by the VPS53 gene. This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi.

Synonyms

Intraflagellar transport protein 88 homolog; Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10; TPR repeat protein 10; IFT88; TG737; TTC10

Gene Name

intraflagellar transport 88

Gene ID

55275

UniProt

Q5VIR6

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human VPS53 recombinant protein (Position: E11-T699).

Clonality

Polyclonal

Tissue Specificity

Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ben-Zeev, B., Hoffman, C., Lev, D., Watemberg, N., Malinger, G., Brand, N., Lerman-Sagie, T. Progressive cerebellocerebral atrophy: a new syndrome with microcephaly, mental retardation, and spastic quadriplegia. J. Med. Genet. 40: e96, 2003. Note: Electronic Article. 2. Brass, A. L., Dykxhoorn, D. M., Benita, Y., Yan, N., Engelman, A., Xavier, R. J., Lieberman, J., Elledge, S. J. Identification of host proteins required for HIV infection through a functional genomic screen. Science 319: 921-926, 2008. 3. Feinstein, M., Flusser, H., Lerman-Sagie, T., Ben-Zeev, B., Lev, D., Agamy, O., Cohen, I., Kadir, R., Sivan, S., Leshinsky-Silver, E., Markus, B., Birk, O. S. VPS53 mutations cause progressive cerebello-cerebral atrophy type 2 (PCCA2). J. Med. Genet. 51: 303-308, 2014.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

104553 MW

Observed Molecular Weight

100 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -