Anti-TTC11/FIS1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-TTC11/FIS1 Antibody Picoband® catalog # A01932-3. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Mitochondrial fission 1 protein (FIS1) is a protein that in humans is encoded by the FIS1 gene on chromosome 7. It is mapped to 7q22.1. The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. Its role in mitochondrial fission thus implicates it in the regulation of mitochondrial morphology, the cell cycle, and apoptosis. By extension, the protein is involved in associated diseases, including neurodegenerative diseases and cancers.

Synonyms

Mitochondrial fission 1 protein; FIS1 homolog; hFis1; Tetratricopeptide repeat protein 11; TPR repeat protein 11; FIS1; TTC11; CGI-135

Gene Name

fission, mitochondrial 1

Gene ID

51024

UniProt

Q9Y3D6

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TTC11/FIS1 recombinant protein (Position: E18-S152).

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Expressed in the rod and cone photoreceptors, extending from the tips of the outer segment (OS) through the inner segment (IS) and outer nuclear layer (ONL) and into the synaptic terminals of the outer plexiform layer (ONL). Also detected in the bipolar, horizontal and amacrine cells in the inner nuclear layer (INL), extending to the inner plexiform layer (IPL) and though the ganglion cell layer (GCL) and into the nerve fiber layer (NFL) (at protein level).

Applications

WB,IHC,ICC,IF,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Dohm, J. A., Lee, S. J., Hardwick, J. M., Hill, R. B., Gittis, A. G. Cytosolic domain of the human mitochondrial fission protein Fis1 adopts a TPR fold. Proteins 54: 153-156, 2004. 2. Frieden, M., James, D., Castelbou, C., Danckaert, A., Martinou, J.-C., Demaurex, N. Ca(2+) homeostasis during mitochondrial fragmentation and perinuclear clustering induced by hFis1. J. Biol. Chem. 279: 22704-22714, 2004. 3. James, D. I., Parone, P. A., Mattenberger, Y., Martinou, J.-C. hFis1, a novel component of the mammalian mitochondrial fission machinery. J. Biol. Chem. 278: 36373-36379, 2003. Note: Erratum: J. Biol. Chem. 279: 36166 only, 2004.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

17 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>