Anti-MASPIN Antibody Picoband® (monoclonal, 7G4E1), APC Conjugate

Boster Bio Anti-MASPIN Antibody Picoband® (monoclonal, 7G4E1) catalog # M03409. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.

Synonyms

Cadherin-3; Placental cadherin; P-cadherin; CDH3; CDHP

Gene Name

cadherin 3, type 1, P-cadherin (placental)

Gene ID

5268

UniProt

P36952

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.

Clonality

Monoclonal

Clone

Clone: 7G4E1

Tissue Specificity

Expressed in some normal epithelial tissues and in some carcinoma cell lines.

Applications

WB,IHC,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Blandamura S, et al. Maspin expression, subcellular localization and clinicopathological correlation in endometrial hyperplasia and endometrial adenocarcinoma. Histol Histopathol, 2014 Jun. 2. Taskiran C, et al. Maspin expression in endometrial hyperplasia and carcinoma, and its relation with angiogenesis. Eur J Gynaecol Oncol, 2014. 3. Triulzi T, et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer, 2014 Jun 15.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

42 kDa

Fragment

Mouse IgG2a

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human