Anti-Ezrin/EZR Antibody Picoband®, APC Conjugate

Boster Bio Anti-Ezrin/EZR Antibody Picoband® catalog # A01750-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Ezrin also known as cytovillin or villin-2 is a protein that in humans is encoded by the EZR gene. The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Synonyms

Dihydropyrimidine dehydrogenase [NADP (+)]; DHPDHase; DPD; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase; DPYD

Gene Name

dihydropyrimidine dehydrogenase

Gene ID

7430

UniProt

P15311

Host

Rabbit

Reactivity

Human,Mouse,Rat,Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Ezrin/EZR, which shares 85.7% amino acid (aa) sequence identity with mouse and rat EZR.

Clonality

Polyclonal

Tissue Specificity

Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.

Applications

Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Allenspach, E. J., Cullinan, P., Tong, J., Tang, Q., Tesciuba, A. G., Cannon, J. L., Takahashi, S. M., Morgan, R., Burkhardt, J. K., Sperling, A. I. ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse. Immunity 15: 739-750, 2001. 2. Bonilha, V. L., Rodriguez-Boulan, E. Polarity and developmental regulation of two PDZ proteins in the retinal pigment epithelium. Invest. Ophthal. Vis. Sci. 42: 3274-3282, 2001. 3. Faure, S., Salazar-Fontana, L. I., Semichon, M., Tybulewicz, V. L. J., Bismuth, G., Trautmann, A., Germain, R. N., Delon, J. ERM proteins regulate cytoskeleton relaxation promoting T cell-APC conjugation. Nature Immun. 5: 272-279, 2004.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

81 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human