Anti-HMGCS1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-HMGCS1 Antibody Picoband® catalog # A05313-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

In molecular biology, hydroxymethylglutaryl-CoA synthase or HMG-CoA synthase EC 2.3.3.10 is an enzyme which catalyzes the reaction in which acetyl-CoA condenses with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Cytoplasmic 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS1) catalyzes the first committed step of mevalonate pathway essential for cholesterol biosynthesis. HMGCS1 transcription is regulated by sterol levels. Studies showed that miR-223 reduces cholesterol biosynthesis by inhibiting HMGCS1 and methylsterol monooxygenase 1. In addition, activation of the EGFR family member ERBB4 induces the expression of SREBP-regulated genes (including HMGCS1) involved in cholesterol biosynthesis, suggesting a role of this enzyme in the metabolic re-programming in ERBB4-driven cancers.

Synonyms

Loricrin; LOR; LRN

Gene Name

loricrin

Gene ID

3157

UniProt

Q01581

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human HMGCS1 recombinant protein (Position: K251-H520).

Clonality

Polyclonal

Tissue Specificity

Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Major keratinocyte cell envelope protein.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ayte, J., Gil-Gomez, G., Haro, D., Marrero, P. F., Hegardt, F. G. Rat mitochondrial and cytosolic 3-hydroxy-3-methylglutaryl-CoA synthases are encoded by two different genes. Proc. Nat. Acad. Sci. 87: 3874-3878, 1990. 2. Gil, G., Smith, J. R., Goldstein, J. L., Brown, M. S. Optional exon in the 5-prime-untranslated region of 3-hydroxy-3-methylglutaryl coenzyme A synthase gene: conserved sequence and splicing pattern in humans and hamsters. Proc. Nat. Acad. Sci. 84: 1863-1866, 1987. 3. Leonard, S., Arbogast, D., Geyer, D., Jones, C., Sinensky, M. Localization of the gene encoding 3-hydroxy-3-methylglutaryl-coenzyme A synthase to human chromosome 5. Proc. Nat. Acad. Sci. 83: 2187-2189, 1986.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

57 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -