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Anti-FOXJ1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-FOXJ1 Antibody Picoband® catalog # A04942-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Forkhead box protein J1 is a protein that in humans is encoded by the FOXJ1 gene. This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.

Synonyms

Meiotic recombination protein REC8 homolog; Cohesin Rec8p; REC8; REC8L1

Gene Name

REC8 meiotic recombination protein

Gene ID

2302

UniProt

Q92949

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human FOXJ1 recombinant protein (Position: D27-A405).

Clonality

Polyclonal

Tissue Specificity

Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

References & Citations

1. Avraham, K. B., Fletcher, C., Overdier, D. G., Clevidence, D. E., Lai, E., Costa, R. H., Jenkins, N. A., Copeland, N. G. Murine chromosomal location of eight members of the hepatocyte nuclear factor 3/fork head winged helix family of transcription factors. Genomics 25: 388-393, 1995. 2. Chen, J., Knowles, H. J., Hebert, J. L., Hackett, B. P. Mutation of the mouse hepatocyte nuclear factor/forkhead homologue 4 gene results in an absence of cilia and random left-right asymmetry. J. Clin. Invest. 102: 1077-1082, 1998. 3. Li, C.-S., Chae, S.-C., Lee, J.-H., Zhang, Q., Chung, H.-T. Identification of single nucleotide polymorphisms in FOXJ1 and their association with allergic rhinitis. J. Hum. Genet. 51: 292-297, 2006.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A04942-3

Product MSDS

https://www.bosterbio.com/msds?sku=A04942-3

Calculated Molecular Weight

56903 MW

Observed Molecular Weight

55 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Nucleus. Chromosome. Centromere.

Protein Name

Vesicular acetylcholine transporter

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