Anti-Coronin 1a/TACO/CORO1A Antibody Picoband®, APC Conjugate

Boster Bio Anti-Coronin 1a/TACO/CORO1A Antibody Picoband® catalog # A04245-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Coronin-1A is a protein that in humans is encoded by the CORO1A gene. This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16.

Synonyms

Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CD23; Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; FCER2; CD23A; CLEC4J; FCE2; IGEBF

Gene Name

Fc fragment of IgE, low affinity II, receptor for (CD23)

Gene ID

11151

UniProt

P31146

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Coronin 1a/TACO/CORO1A recombinant protein (Position: E173-K461).

Clonality

Polyclonal

Tissue Specificity

Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

References & Citations

1. Ferrari, G., Langen, H., Naito, M., Pieters, J. A coat protein on phagosomes involved in the intracellular survival of mycobacteria. Cell 97: 435-447, 1999. 2. Foger, N., Rangell, L., Danilenko, D. M., Chan, A. C. Requirement for coronin 1 in T lymphocyte trafficking and cellular homeostasis. Science 313: 839-842, 2006. 3. Haraldsson, M. K., Louis-Dit-Sully, C. A., Lawson, B. R., Sternik, G., Santiago-Raber, M.-L., Gascoigne, N. R. J., Theofilopoulos, A. N., Kono, D. H. The lupus-related Lmb3 locus contains a disease-suppressing coronin-1A gene mutation. Immunity 28: 40-51, 2008.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

57 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -