Anti-Carbonic Anhydrase I/CA1 Picoband® Antibody (monoclonal, 4D5), APC Conjugate

Boster Bio Anti-Carbonic Anhydrase I/CA1 Picoband® Antibody (monoclonal, 4D5) catalog # M00170-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.

Synonyms

Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter; SLC2A1; GLUT1

Gene Name

solute carrier family 2 (facilitated glucose transporter), member 1

Gene ID

759

UniProt

P00915

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CA1 recombinant protein (Position: D9-F261). Human CA1 shares 78.5% and 81% amino acid (aa) sequence identity with mouse and rat CA1, respectively.

Clonality

Monoclonal

Clone

Clone: 4D5

Tissue Specificity

Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.

Applications

Flow Cytometry,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Lowe N, Edwards YH, Edwards M, Butterworth PH (Nov 1991). "Physical mapping of the human carbonic anhydrase gene cluster on chromosome 8". Genomics 10 (4): 882–8. 2. "Entrez Gene: CA1 carbonic anhydrase I".

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46659 MW

Observed Molecular Weight

29 kDa

Fragment

Mouse IgG2a

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human