Anti-FEZF1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-FEZF1 Antibody Picoband® catalog # A09653-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

FEZ family zinc finger 1 is a protein that in humans is encoded by the FEZF1 gene. This gene encodes a transcriptional repressor that belongs to the zinc finger double domain protein family. The encoded protein is thought to play a role in the embryonic migration of gonadotropin-releasing hormone neurons into the brain. Mutations in this gene are associated with hypogonadotropic hypogonadism-22 with anosmia. Alternative splicing results in multiple transcript variants.

Synonyms

One cut domain family member 2; Hepatocyte nuclear factor 6-beta; HNF-6-beta; One cut homeobox 2; Transcription factor ONECUT-2; OC-2; ONECUT2; HNF6B

Gene Name

one cut homeobox 2

Gene ID

389549

UniProt

A0PJY2

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human FEZF1 recombinant protein (Position: A45-K258).

Clonality

Polyclonal

Tissue Specificity

Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Applications

ELISA,Flow Cytometry,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

References & Citations

1. Hirata, T., Nakazawa, M., Muraoka, O., Nakayama, R., Suda, Y., Hibi, M. Zinc-finger genes Fez and Fez-like function in the establishment of diencephalon subdivisions. Development 133: 3993-4004, 2006. 2. Kotan, L. D., Hutchins, B. I., Ozkan, Y., Demirel, F., Stoner, H., Cheng, P. J., Esen, I., Gurbuz, F., Bicakci, Y. K., Mengen, E., Yuksel, B., Wray, S., Topaloglu, A. K. Mutations in FEZF1 cause Kallmann syndrome. Am. J. Hum. Genet. 95: 326-331, 2014. 3. Song, I.-S., Oh, N. S., Kim, H.-T., Ha, G.-H., Jeong, S.-Y., Kim, J.-M., Kim, D.-I., Yoo, H.-S., Kim, C.-H., Kim, N.-S. Human ZNF312b promotes the progression of gastric cancer by transcriptional activation of the K-ras gene. Cancer Res. 69: 3131-3139, 2009. Note: Erratum: Cancer Res. 69: 4092 only, 2009.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

65 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -