Anti-FADD Antibody Picoband®, APC Conjugate

FADD, Fas-Associated protein with Death Domain, is a universal adaptor protein in apoptosis that mediates signaling of all known death domain-containing members of the TNF receptor superfamily. The FADD gene contains 2 exons and spans approximately 3.6 kb. By analysis of somatic cell hybrid panels and by fluorescence in situ hybridization, the FADD gene is mapped to 11q13.3. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, thus, it participates in the death signaling initiated by these receptors.

A synthetic peptide corresponding to a sequence at the C-terminus of mouse FADD, which shares 63.6% and 81.8% amino acid (aa) sequence identity with human and rat FADD, respectively.

1. Kabra, N. H., Kang, C., Hsing, L. C., Zhang, J., Winoto, A.T cell-specific FADD-deficient mice: FADD is required for early T cell development.Proc. Nat. Acad. Sci. 98: 6307-6312, 2001. 2. Kim, P. K. M., Dutra, A. S., Chandrasekharappa, S. C., Puck, J. M.Genomic structure and mapping of human FADD, an intracellular mediator of lymphocyte apoptosis.J. Immun. 157: 5461-5466, 1996. 3. Lee, H.-J., Pyo, J.-O., Oh, Y., Kim, H.-J., Hong, S., Jeon, Y.-J., Kim, H., Cho, D.-H., Woo, H.-N., Song, S., Nam, J.-H., Kim, H. J., Kim, K.-S., Jung, Y.-K.AK2 activates a novel apoptotic pathway through formation of a complex with FADD and caspase-10.Nature Cell Biol. 9: 1303-1310, 2007.

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Western blot, 0.1-0.25μg/ml, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse<br>