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Anti-CEBP Delta/CEBPD Antibody Picoband®, APC Conjugate

Boster Bio Anti-CEBP Delta/CEBPD Antibody Picoband® catalog # A03499-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

CCAAT/enhancer-binding protein delta is a protein that in humans is encoded by the CEBPD gene. The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. The cytogenetic location of this locus has been reported as both 8p11 and 8q11.

Synonyms

Calpain-2 catalytic subunit;3.4.22.53;Calcium-activated neutral proteinase 2;CANP 2;Calpain M-type;Calpain large polypeptide L2;Calpain-2 large subunit;Millimolar-calpain;M-calpain;CAPN2;CANPL2;

Gene Name

Calpain-2 catalytic subunit

Gene ID

1052

UniProt

P49716

Host

Rabbit

Reactivity

Human,Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CEBP Delta/CEBPD recombinant protein (Position: K213-R269).

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). .

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Barbaro, V., Testa, A., Di Iorio, E., Mavilio, F., Pellegrini, G., De Luca, M. C/EBP-delta regulates cell cycle and self-renewal of human limbal stem cells. J. Cell Biol. 177: 1037-1049, 2007. 2. Cleutjens, C. B. J. M., van Eekelen, C. C. E. M., van Dekken, H., Smit, E. M. E., Hagemeijer, A., Wagner, M. J., Wells, D. E., Trapman, J. The human C/EBP-delta (CRP3/CELF) gene: structure and chromosomal localization. Genomics 16: 520-523, 1993. 3. Gigliotti, A. P., Johnson, P. F., Sterneck, E., DeWille, J. W. Nulliparous CCAAT/enhancer binding protein-delta (C/EBP-delta) knockout mice exhibit mammary gland ductal hyperlasia (sic). Exp. Biol. Med. 228: 278-285, 2003.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A03499-4

Product MSDS

https://www.bosterbio.com/msds?sku=A03499-4

Calculated Molecular Weight

79995 MW

Observed Molecular Weight

36 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca (2+) binding.

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