Anti-OTULIN Antibody Picoband®, APC Conjugate

Boster Bio Anti-OTULIN Antibody Picoband® catalog # A07938-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a member of the peptidase C65 family of ubiquitin isopeptidases. Members of this family remove ubiquitin from proteins. The encoded enzyme specifically recognizes and removes M1 (Met1)-linked, or linear, ubiquitin chains from protein substrates. Linear ubiquitin chains are known to regulate the NF-kappa B signaling pathway in the context of immunity and inflammation. Mutations in this gene cause a potentially fatal autoinflammatory syndrome in human patients.

Synonyms

Glycerol-3-phosphate dehydrogenase 1-like protein; GPD1-L; GPD1L; KIAA0089

Gene Name

glycerol-3-phosphate dehydrogenase 1 like

Gene ID

90268

UniProt

Q96BN8

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human OTULIN recombinant protein (Position: A49-L352).

Clonality

Polyclonal

Tissue Specificity

Most highly expressed in heart tissue, with lower levels in the skeletal muscle, kidney, lung and other organs.

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD (H) balance induced by DPD1L.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Damgaard, R. B., Walker, J. A., Marco-Casanova, P., Morgan, N. V., Titheradge, H. L., Elliott, P. R., McHale, D., Maher, E. R., McKenzie, A. N. J., Komander, D. The deubiquitinase OTULIN is an essential negative regulator of inflammation and autoimmunity. Cell 166: 1215-1230, 2016. 2. Gross, M. B. Personal Communication. Baltimore, Md. 3/25/2014. 3. Heger, K., Wickliffe, K. E., Ndoja, A., Zhang, J., Murthy, A., Dugger, D. L., Maltzman, A., de Sousa E Melo, F., Hung, J., Zeng, Y., Verschueren, E., Kirkpatrick, D. S., and 10 others. OTULIN limits cell death and inflammation by deubiquitinating LUBAC. Nature 559: 120-124, 2018.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

49229 MW

Observed Molecular Weight

43-45 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human ELISA, 0.1-0.5μg/ml, -