Anti-GNG4 Antibody Picoband®, APC Conjugate

Boster Bio Anti-GNG4 Antibody Picoband® catalog # A13925-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Guanine nucleotide-binding protein G (I)/G (S)/G (O) subunit gamma-4 is a protein that in humans is encoded by the GNG4 gene. This gene encodes the gamma subunit of the heterotrimeric G-proteins that are comprised of alpha, beta and gamma subunits. Upon activation by G protein-coupled receptors, the beta-gamma heterodimer dissociates from the alpha subunit to activate downstream signaling events. Alternate splicing results in multiple transcript variants.

Synonyms

RNA-binding protein 47;RNA-binding motif protein 47;RBM47;

Gene Name

RNA-binding protein 47

Gene ID

2786

UniProt

P50150

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human GNG4 recombinant protein (Position: M1-D52).

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Ahmad, W., Li, S., Chen, H., Tuck-Muller, C. M., Pittler, S. J., Aronson, N. N., Jr. Lysosomal chitobiase (CTB) and the G-protein gamma-5 subunit (GNG5) genes co-localize to human chromosome 1p22. Cytogenet. Cell Genet. 71: 44-46, 1995. 2. Gilman, A. G. G proteins: transducers of receptor-generated signals. Annu. Rev. Biochem. 56: 615-649, 1987. 3. Ray, K., Kunsch, C., Bonner, L. M., Robishaw, J. D. Isolation of cDNA clones encoding eight different human G protein gamma subunits, including three novel forms designated the gamma-4, gamma-10, and gamma-11 subunits. J. Biol. Chem. 270: 21765-21771, 1995.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

64099 MW

Observed Molecular Weight

12 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -