Anti-MAP4K5 Antibody Picoband®, APC Conjugate

Boster Bio Anti-MAP4K5 Antibody Picoband® catalog # A10186-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Mitogen-activated protein kinase kinase kinase kinase 5 is an enzyme that in humans is encoded by the MAP4K5 gene. This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Synonyms

Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3;

Gene Name

Protein NDRG3

Gene ID

11183

UniProt

Q9Y4K4

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MAP4K5, which shares 94.4% amino acid (aa) sequence identity with both mouse and rat MAP4K5.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Highly expressed in brain. .

Applications

IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Tung, R. M., Blenis, J. A novel human SPS1/STE20 homologue, KHS, activates Jun N-terminal kinase. Oncogene 14: 653-659, 1997.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

41409 MW

Observed Molecular Weight

95 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br>