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Anti-CDC123 Antibody Picoband®, APC Conjugate

Boster Bio Anti-CDC123 Antibody Picoband® catalog # A08251-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Cell division cycle protein 123 homolog is a protein that in humans is encoded by the CDC123 gene. It is mapped to chromosome 10p14-p13. CDC123 is predicted to function as an assembly factor for eukaryotic translation initiation factor-2, which recruits initiator met-tRNA to the 40S ribosome to begin protein synthesis.

Synonyms

Neuropeptides B/W receptor type 1; G-protein coupled receptor 7; NPBWR1; GPR7

Gene Name

neuropeptides B and W receptor 1

Gene ID

8872

UniProt

O75794

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CDC123 recombinant protein (Position: M1-D336).

Clonality

Polyclonal

Tissue Specificity

Found in cerebellum and frontal cortex. Detected at high levels in hippocampus, amygdala and trachea; at moderate levels in fetal brain, pituitary gland and prostate. Not in caudate, accumbens, kidney or liver. Also detected at high levels in lung carcinoma.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Has a higher affinity for neuropeptide B.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 9/29/2017. 2. Okuda, A., Kimura, G. An amino acid change in novel protein D123 is responsible for temperature-sensitive G1-phase arrest in a mutant of rat fibroblast line 3Y1. Exp. Cell Res. 223: 242-249, 1996. 3. Onisto, M., Zeilante, P., Scannapieco, P., Pellati, D., Pozza, M., Caenazzo, C., Negro, A., Garbisa, S. Expression study on D123 gene product: evidence for high positivity in testis. Exp. Cell Res. 242: 451-459, 1998.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

45 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Cell membrane. Multi-pass membrane protein.

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