Anti-Rad9b Antibody Picoband®, APC Conjugate

Boster Bio Anti-Rad9b Antibody Picoband® catalog # A13507-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

By coimmunoprecipitation of erythroleukemia cells transfected with RAD9B, it was found that RAD9B associated with 3 endogenous checkpoint proteins, HUS1, RAD1, and RAD17, possibly in a 9-1-1-like complex. RAD9B also interacted with transfected HUS1B. It was also found decreased abundance of RAD9B mRNA in testicular seminomas relative to normal tissue controls and other types of testicular tumors.

Synonyms

RNA-binding protein 47;RNA-binding motif protein 47;RBM47;

Gene Name

RNA-binding protein 47

Gene ID

231724

UniProt

Q6WBX7

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived mouse Rad9b recombinant protein (Position: M1-G403).

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

ELISA,Flow Cytometry,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Dufault, V. M., Oestreich, A. J., Vroman, B. T., Karnitz, L. M. Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene. Genomics 82: 644-651, 2003. 2. Hopkins, K. M., Wang, X., Berlin, A., Hang, H., Thaker, H. M., Lieberman, H. B. Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue. Cancer Res. 63: 591-598, 2003.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

64099 MW

Observed Molecular Weight

60 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse ELISA, 0.1-0.5μg/ml, -