Anti-mGluR1/GRM1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-mGluR1/GRM1 Antibody Picoband® catalog # A03049-4. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms

Calbindin; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type; CALB1; CAB27

Gene Name

calbindin 1, 28kDa

Gene ID

2911

UniProt

Q13255

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human mGluR1/GRM1 recombinant protein (Position: R25-E466).

Clonality

Polyclonal

Tissue Specificity

Detected in skin. In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells. Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Buffers cytosolic calcium. May stimulate a membrane Ca (2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Ganesh, S., Amano, K., Yamakawa, K. Assignment of the gene GRM1 coding for metabotropic glutamate receptor 1 to human chromosome band 6q24 by in situ hybridization. Cytogenet. Cell Genet. 88: 314-315, 2000. 2. Ortiz, P., Vanaclocha, F., Lopez-Bran, E., Esquivias, J. I., Lopez-Estebaranz, J. L., Martin-Gonzalez, M., Arrue, I., Garcia-Romero, D., Ochoa, C., Gonzalez-Perez, A., Ruiz, A., Real, L. M. Genetic analysis of the GRM1 gene in human melanoma susceptibility. Europ. J. Hum. Genet. 15: 1176-1182, 2007. 3. Sachs, A. J., Schwendinger, J. K., Yang, A. W., Haider, N. B., Nystuen, A. M. The mouse mutants recoil wobbler and nmf373 represent a series of Grm1 mutations. Mammalian Genome 18: 749-756, 2007.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

132 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse ELISA, 0.1-0.5μg/ml, -