Anti-CD22 Antibody Picoband® (monoclonal, 5E7), APC Conjugate

Boster Bio Anti-CD22 Antibody Picoband® (monoclonal, 5E7) catalog # M01572-2. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.

Synonyms

B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD22; SIGLEC2

Gene Name

CD22 molecule

Gene ID

933

UniProt

P20273

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD22, different from the related mouse sequence by ten amino acids.

Clonality

Monoclonal

Clone

Clone: 5E7

Tissue Specificity

B-lymphocytes.

Applications

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. John, B.; Herrin, B. R.; Raman, C.; Wang, Y.; Bobbitt, K. R.; Brody, B. A.; Justement, L. B. The B cell coreceptor CD22 associates with AP50, a clathrin-coated pit adapter protein, via tyrosine-dependent interaction. J. Immun. 170: 3534-3543, 2003. 2. O'Keefe, T. L.; Williams, G. T.; Davies, S. L.; Neuberger, M. S. Hyperresponsive B cells in CD22-deficient mice. Science 274: 798-801, 1996. 3. Wilson, G. L.; Najfeld, V.; Kozlow, E.; Menniger, J.; Ward, D.; Kehrl, J. H. Genomic structure and chromosomal mapping of the human CD22 gene. J. Immun. 150: 5013-5024, 1993.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.