Anti-CETP Antibody Picoband®, APC Conjugate

Boster Bio Anti-CETP Antibody Picoband® catalog # A00309-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

CETP (Cholesteryl Ester Transfer Protein Plasma), is a plasma protein that facilitates the transport of cholesteryl esters and triglycerides between the lipoproteins. CETP is also known as lipid transfer protein I (Day et al., 1994). Sparkes et al. (1987) used a CETP probe against DNA from a human/mouse somatic cell hybrid panel to assign the CETP gene to chromosome 16. Because the role of CETP in atherosclerosis remained unclear, Okamoto et al. (2000) attempted to develop a potent, specific CETP inhibitor. One inhibitor, JTT-705, forms a disulfide bond with CETP and increases high density lipoprotein (HDL) cholesterol, decreases non-HDL cholesterol, and inhibits the progression of atherosclerosis in rabbits.

Synonyms

Cholesteryl ester transfer protein; Lipid transfer protein I; CETP

Gene Name

cholesteryl ester transfer protein

Gene ID

1071

UniProt

P11597

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CETP recombinant protein (Position: H77-K353).

Clonality

Polyclonal

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL (PubMed:3600759, PubMed:24293641). Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination (PubMed:17237796).

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Day, J. R., Albers, J. J., Lofton-Day, C. E., Gilbert, T. L., Ching, A. F. T., Grant, F. J., O'Hara, P. J., Marcovina, S. M., Adolphson, J. L. Complete cDNA encoding human phospholipid transfer protein from human endothelial cells. J. Biol. Chem. 269: 9388-9391, 1994. 2. Okamoto, H., Yonemori, F., Wakitani, K., Minowa, T., Maeda, K., Shinkai, H. A cholesteryl ester transfer protein inhibitor attenuates atherosclerosis in rabbits. Nature 406: 203-207, 2000. 3. Sparkes, R. S., Drayna, D., Mohandas, T., Klisak, I., Heinzmann, C., Lawn, R., Lusis, A. J. Assignment of cholesterol ester transfer protein (CETP) gene to human 16q21. (Abstract) Cytogenet. Cell Genet. 46: 696 only, 1987.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

63525 MW

Observed Molecular Weight

75 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Isoform 1: Cell membrane ; Single- pass type I membrane protein . Late endosome . Lysosome . Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. .