Anti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2), APC Conjugate

Boster Bio Anti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) catalog # M01163-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Synonyms

Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK; BCL2L7; CDN1

Gene Name

BCL2 antagonist/killer 1

Gene ID

578

UniProt

Q16611

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human BAK/BAK1 recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK.

Clonality

Monoclonal

Clone

Clone: 4C2

Tissue Specificity

Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.

Applications

Flow Cytometry,IF,IHC,ICC,WB

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Plays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Chittenden, T., Harrington, E. A., O'Connor, R., Flemington, C., Lutz, R. J., Evan, G. I., Guild, B. C.Induction of apoptosis by the Bcl-2 homologue Bak.Nature 374: 733-736, 1995. 2. Herberg, J. A., Phillips, S., Beck, S., Jones, T., Sheer, D., Wu, J. J., Prochazka, V., Barr, P. J., Kiefer, M. C., Trowsdale, J.Genomic structure and domain organisation of the human Bak gene.Gene 211: 87-94, 1998. 3. Kiefer, M. C., Brauer, M. J., Powers, V. C., Wu, J. J., Umansky, S. R., Tomei, L. D., Barr, P. J.Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.Nature 374: 736-739, 1995.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

25 kDa

Fragment

Mouse IgG2b

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells