Anti-ROBO1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-ROBO1 Antibody Picoband® catalog # A01530-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Roundabout homolog 1 is a protein that in humans is encoded by the ROBO1 gene. Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1

Gene Name

roundabout guidance receptor 1

Gene ID

6091

UniProt

Q9Y6N7

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ROBO1 recombinant protein (Position: F264-P897).

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with exception of kidney.

Applications

ELISA,Flow Cytometry,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. "Entrez Gene: ROBO1 roundabout, axon guidance receptor, homolog 1 (Drosophila)". 2. Hannula-Jouppi K, Kaminen-Ahola N, Taipale M, Eklund R, Nopola-Hemmi J, Kääriäinen H, Kere J (October 2005). "The axon guidance receptor gene ROBO1 is a candidate gene for developmental dyslexia". PLoS Genet. 1 (4): e50.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

180,930 MW

Observed Molecular Weight

250 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells ELISA, 0.1-0.5μg/ml

Subcellular Location

Cell membrane. Single-pass type I membrane protein. Axon. Endoplasmic reticulum-Golgi intermediate compartment membrane. Single-pass membrane protein.