Anti-Annexin A3/ANXA3 Antibody Picoband®, APC Conjugate

Boster Bio Anti-Annexin A3/ANXA3 Antibody Picoband® catalog # A04796. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Annexin A3 is a protein that in humans is encoded by the Annexin A3 gene. The Annexin A3 gene contains 13 exons and spans 58 kb of genomic DNA. The Annexin A3 gene is mapped to 4q21. It is abnormally expressed in fetuses of both IVF and ICSI, which may contribute to the increase risk of birth defects in these ART. This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.

Synonyms

Annexin A3; 35-alpha calcimedin; Annexin III; Annexin-3; Inositol 1,2-cyclic phosphate 2-phosphohydrolase; Lipocortin III; Placental anticoagulant protein III;PAP-III; ANXA3; ANX3

Gene Name

annexin A3

Gene ID

306

UniProt

P12429

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Annexin A3/ANXA3 recombinant protein (Position: D23-D323).

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Highly expressed in ovary followed by spleen, thymus, prostate, testes and large intestine. Weakly expressed in small intestine.

Applications

ELISA,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Bianchi C, et al. Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3. Am J Pathol, 2010 Apr. 2. Jung EJ, et al. Decreased annexin A3 expression correlates with tumor progression in papillary thyroid cancer. Proteomics Clin Appl, 2010 May. 3. Yan X, et al. Increased expression of annexin A3 is a mechanism of platinum resistance in ovarian cancer. Cancer Res, 2010 Feb 15.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

36 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> ELISA, 0.1-0.5μg/ml<br>

Subcellular Location

Cytosol. Extracellular exosome. Plasma membrane. Axon. Cytoplasm. Dendrite. Membrane. Neuronal cell body. Phagocytic vesicle membrane. Specific granule.