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Anti-Leucyl tRNA synthetase /LARS Antibody Picoband®, APC Conjugate

Boster Bio Anti-Leucyl tRNA synthetase /LARS Antibody Picoband® catalog # A01109. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Leucyl-tRNA synthetase, cytoplasmic is an enzyme that in humans is encoded by the LARS gene. This gene is mapped to 5q32. It encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA (Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. A mutation in this gene was found in affected individuals with infantile liver failure syndrome 1. Alternatively spliced transcript variants of this gene have been observed.

Synonyms

Leucine--tRNA ligase, cytoplasmic; Leucyl-tRNA synthetase; LeuRS; LARS; KIAA1352

Gene Name

leucyl-tRNA synthetase

Gene ID

51520

UniProt

Q9P2J5

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Leucyl tRNA synthetase/LARS recombinant protein (Q628-Q855).

Clonality

Polyclonal

Tissue Specificity

Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.

Applications

ELISA,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

References & Citations

1. Casey, J. P., McGettigan, P., Lynam-Lennon, N., McDermott, M., Regan, R., Conroy, J., Bourke, B., O'Sullivan, J., Crushell, E., Lynch, S., Ennis, S. Identification of a mutation in LARS as a novel cause of infantile hepatopathy. Molec. Genet. Metab. 106: 351-358, 2012. 2. Dana, S., Wasmuth, J. J. Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5. Molec. Cell. Biol. 2: 1220-1228, 1982. 3. Gerken, S. C., Wasmuth, J. J., Arfin, S. M. Threonyl-rRNA synthetase gene maps close to leucyl-tRNA synthetase gene on human chromosome 5. Somat. Cell Molec. Genet. 12: 519-522, 1986.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51735 MW

Observed Molecular Weight

134 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml<br> ELISA, 0.1-0.5μg/ml<br>

Subcellular Location

Cytoplasm.

Protein Name

Putative Polycomb group protein ASXL1

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