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Anti-Bid Antibody Picoband® (APC Conjugate)

Boster Bio Anti-Bid Antibody Picoband® catalog # A00730. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, the human BID gene is mapped to 22q11. It is reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Synonyms

BH3-interacting domain death agonist;p22 BID;BID;BH3-interacting domain death agonist p15;p15 BID;BH3-interacting domain death agonist p13;p13 BID;BH3-interacting domain death agonist p11;p11 BID;Bid;

Gene Name

BH3-interacting domain death agonist

UniProt

P70444

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Bid recombinant protein (Position: M1-D195). Mouse Bid shares 64.6% and 87.2% amino acid (aa) sequence identity with human and rat Bid, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Applications

Flow Cytometry,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A00730

Product MSDS

https://www.bosterbio.com/msds?sku=A00730

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat, By Heat<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Mouse<br>

Subcellular Location

Cytoplasm . Mitochondrion membrane . When uncleaved, it is predominantly cytoplasmic. p15 BID translocates to mitochondria as an integral membrane protein. p13 and p22 BID are associated with the mitochondrial membrane.
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