Anti-ACSL1 Antibody Picoband® (APC Conjugate)

Long-chain-fatty-acid—CoA ligase 1 is an enzyme that in humans is encoded by the ACSL1 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. This specific protein is most commonly found in mitochondria and peroxisomes.

Long-chain-fatty-acid--CoA ligase 1;6.2.1.3;Acyl-CoA synthetase 1;ACS1;Long-chain acyl-CoA synthetase 1;LACS 1;Long-chain acyl-CoA synthetase 2;LACS 2;Long-chain fatty acid-CoA ligase 2;Palmitoyl-CoA ligase 1;Palmitoyl-CoA ligase 2;ACSL1;FACL1, FACL2, LACS, LACS1, LACS2;

E. coli-derived human ACSL1 recombinant protein (Position: D604-V698). Human ACSL1 shares 81.1% and 86.3% amino acid (aa) sequence identity with mouse and rat ACSL1, respectively.

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human<br>

Mitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein . Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .