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Anti-DDAH2 Antibody Picoband® (APC Conjugate)

Boster Bio Anti-DDAH2 Antibody Picoband® catalog # PB10001. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

DDAH2 is known as dimethylarginine dimethylaminohydrolase 2 which is mapped to 6p21.3 by radiation hybrid and FISH analysis. This gene encodes a dimethylarginine dimethylaminohydrolase. DDAH2 functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.

Synonyms

N (G),N (G)-dimethylarginine dimethylaminohydrolase 2;DDAH-2;Dimethylarginine dimethylaminohydrolase 2;3.5.3.18;DDAHII;Dimethylargininase-2;Protein G6a;S-phase protein;DDAH2;DDAH, G6A, NG30;

Gene Name

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

UniProt

O95865

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DDAH2, different from the related mouse and rat sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain. .

Applications

Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Hydrolyzes N (G),N (G)-dimethyl-L-arginine (ADMA) and N (G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat<br> Immunocytochemistry/Immunofluorescence, 2μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human

Subcellular Location

Cytoplasm . Mitochondrion . Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Frequently Asked Questions

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