Anti-IGFBP5 Antibody Picoband® (APC Conjugate)

Boster Bio Anti-IGFBP5 Antibody Picoband® catalog # PB9711. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Insulin-like growth factor-binding protein 5?is a?protein?that in humans is encoded by the?IGFBP5?gene. The expression of?IGFBP5?by stable transfection and adenovirus-mediated infection is inhibitory to growth in 2 human breast cancer cell lines.?IGFBP5?expression leads to G2/M cell cycle arrest and apoptosis. Stable expression of?IGFBP5?in the breast cancer cell lines also inhibits the formation and growth of tumors following injection in athymic mice. It is concluded that IGFBP5?is a growth inhibitor and proapoptotic agent in breast cancer cells. Additionally, IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. It has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21?kDa fragment (1,?2).

Synonyms

Insulin-like growth factor-binding protein 5;IBP-5;IGF-binding protein 5;IGFBP-5;IGFBP5;IBP5;

Gene Name

Insulin-like growth factor-binding protein 5

UniProt

P24593

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human IGFBP5, different from the related mouse and rat sequences by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Osteosarcoma, and at lower levels in liver, kidney and brain.

Applications

ELISA,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.