Anti-TRAM2 Antibody Picoband®, FITC Conjugate

Boster Bio Anti-TRAM2 Antibody catalog # PA2011. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

TRAM2 (Translocation-Associating Membrane Protein 2), also called KIAA0057, is a protein that in humans is encoded by the TRAM2 gene. TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane. By genomic sequence analysis, Onuchic et al. (1999) mapped the TRAM2 gene to chromosome 6p21.1-p12 near the PKHD1 gene. Upon activation, quiescent hepatic stellate cells proliferate, change morphologically into myofibroblasts, and increase their synthesis of extracellular matrix proteins. Stefanovic et al. (2004) demonstrated that both TRAM2 and collagen type I are upregulated in activated rat and human hepatic stellate cells.

Synonyms

Translocating chain-associated membrane protein 2;Tram2;

Gene Name

Translocating chain-associated membrane protein 2

UniProt

Q924Z5

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRAM2, identical to the related rat sequence, and different from the related human sequence by one amino acid.

Clonality

Polyclonal

Applications

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Necessary for collagen type I synthesis. May couple the activity of the ER Ca (2+) pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca (2+) concentration at the site of collagen synthesis, and a high Ca (2+) concentration may be necessary for the function of molecular chaperones involved in collagen folding (By similarity). .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Nomura, N., Nagase, T., Miyajima, N., Sazuka, T., Tanaka, A., Sato, S., Seki, N., Kawarabayasi, Y., Ishikawa, K., Tabata, S. Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 1: 223-229, 1994. 2. Onuchic, L. F., Mrug, M., Lakings, A. L., Muecher, G., Becker, J., Zerres, K., Avner, E. D., Dixit, M., Somlo, S., Germino, G. G., Guay-Woodford, L. M. Genomic organization of the KIAA0057 gene that encodes a TRAM-like protein and its exclusion as a polycystic kidney and hepatic disease 1 (PKHD1) candidate gene. Mammalian Genome 10: 1175-1178, 1999. 3. Stefanovic, B., Stefanovic, L., Schnabl, B., Bataller, R., Brenner, D. A. TRAM2 protein interacts with endoplasmic reticulum Ca(2+) pump Serca2b and is necessary for collagen type I synthesis. Molec. Cell. Biol. 24: 1758-1768, 2004.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.