Anti-liver Arginase/ARG1 Antibody Picoband®, FITC Conjugate

Boster Bio Anti-liver Arginase/ARG1 Antibody catalog # PA1783. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al. (1987) isolated and characterized a cDNA corresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Arginase-1;3.5.3.1;Liver-type arginase;Type I arginase;Arg1;

Gene Name

Arginase-1

UniProt

P07824

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat liver Arginase, different from the related mouse sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Detected in liver (at protein level). .

Applications

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Cardoso, M. L., Martins, E., Vasconcelos, R., Vilarinho, L., Rocha, J. Identification of a novel R21X mutation in the liver-type arginase gene (ARG1) in four Portuguese patients with argininemia. Hum. Mutat. 14: 355-356, 1999. 2. Grody, W. W., Dodson, A., Klein, D., Kern, R. M., Bassand, P., Cederbaum, S. D. Molecular genetic study of human arginase deficiency. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A191 only, 1989. 3. Haraguchi, Y., Takiguchi, M., Amaya, Y., Kawamoto, S., Matsuda, I., Mori, M. Molecular cloning and nucleotide sequence of cDNA for human liver arginase. Proc. Nat. Acad. Sci. 84: 412-415, 1987.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.