Anti-HDJ2/DNAJA1 Antibody Picoband®, FITC Conjugate

Boster Bio Anti-HDJ2/DNAJA1 Antibody catalog # PA1597. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

DNAJA1 (DnaJ homolog subfamily A member 1), also called DJA1, HSDJ, HSPF4, is a protein that in humans is encoded by the DNAJA1 gene. The deduced 397-amino acid DNAJA1 protein is 32% identical to E. coli DnaJ, with the highest identity in the N-terminal region. Among the known DNAJ homologs in S. cerevisiae, DNAJA1 is most identical to YDJ1, which may be involved in the transport of certain proteins into the mitochondria and endoplasmic reticulum. Dja1 is expressed in mouse testis and prostate by immunohistochemical analysis and Western blot analysis. Transplantation experiments with fluorescence-tagged spermatogonia into Dja1 -/- mice revealed a primary defect of Sertoli cells in maintaining spermiogenesis at steps 8 and 9.

Synonyms

DnaJ homolog subfamily A member 1;DnaJ protein homolog 2;HSDJ;Heat shock 40 kDa protein 4;Heat shock protein J2;HSJ-2;Human DnaJ protein 2;hDj-2;DNAJA1;DNAJ2, HDJ2, HSJ2, HSPF4;

Gene Name

DnaJ homolog subfamily A member 1

UniProt

P31689

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HDJ2, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver. .

Applications

IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co- chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202). .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Chellaiah, A., Davis, A., Mohanakumar, T. Cloning of a unique human homologue of the Escherichia coli DNAJ heat shock protein. Biochim. Biophys. Acta 1174: 111-113, 1993. 2. Banasavadi-Siddegowda YK et al. FKBP38 Peptidylprolyl Isomerase Promotes the Folding of Cystic Fibrosis Transmembrane Conductance Regulator in the Endoplasmic Reticulum. J Biol Chem 286:43071-80 (2011). 3. Chellaiah A, Davis A, Mohanakumar T (Aug 1993). "Cloning of a unique human homologue of the Escherichia coli DNAJ heat shock protein". Biochim Biophys Acta 1174 (1): 111–3.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44868 MW

Observed Molecular Weight

45 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br>

Subcellular Location

Membrane ; Lipid-anchor . Cytoplasm . Microsome . Nucleus . Cytoplasm, perinuclear region . Mitochondrion . Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei. .