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Anti-RHBG Antibody Picoband®, FITC Conjugate

Boster Bio Anti-RHBG Antibody Picoband® catalog # A05585-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Rh family, B glycoprotein, also known as RHBG, is an ammonia transporter protein which in humans is encoded by the RHBG gene. This gene encodes one of two non-erythroid members of the Rhesus (Rh) protein family. Non-erythroid Rh protein family members are mainly expressed in the kidney and belong to the methylammonium-ammonium permease/ammonia transporters superfamily. All Rh family proteins are predicted to be transmembrane proteins with 12 membrane spanning domains and intracytoplasmic N- and C-termini. Alternative splicing of this gene results in multiple transcript variants.

Gene Name

RHBG

Gene ID

57127

UniProt

Q9H310

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human RHBG recombinant protein (Position: R7-L394). Human RHBG shares 87.2% amino acid (aa) sequence identity with mouse and rat RHBG.

Clonality

Polyclonal

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Khademi, S., O'Connell, J., III, Remis, J., Robles-Colmenares, Y., Miercke, L. J. W., Stroud, R. M. Mechanism of ammonia transport by Amt/MEP/Rh: Structure of AmtB at 1.35 A. Science 305: 1587-1594, 2004. 2. Liu, Z., Peng, J., Mo, R., Hui, C., Huang, C.-H. Rh type B glycoprotein is a new member of the Rh superfamily and a putative ammonia transporter in mammals. J. Biol. Chem. 276: 1424-1433, 2001.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

49 kDa

Observed Molecular Weight

47 kDa

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

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