Anti-NUDT6 Antibody Picoband®, FITC Conjugate

Boster Bio Anti-NUDT6 Antibody Picoband® catalog # A08301-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nucleoside diphosphate-linked moiety X motif 6 is a protein that in humans is encoded by the NUDT6 gene. This gene overlaps and lies on the opposite strand from FGF2 gene, and is thought to be the FGF2 antisense gene. The two genes are independently transcribed, and their expression shows an inverse relationship, suggesting that this antisense transcript may regulate FGF2 expression. This gene has also been shown to have hormone-regulatory and antiproliferative actions in the pituitary that are independent of FGF2 expression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Histone-lysine N-methyltransferase SETDB2; Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2; SETDB2; C13orf4; CLLD8; KMT1F

Gene Name

SET domain bifurcated histone lysine methyltransferase 2

Gene ID

11162

UniProt

P53370

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NUDT6 recombinant protein (Position: D45-K303).

Clonality

Polyclonal

Tissue Specificity

Highly expressed in reproductive organs, such as testis, ovary and prostate.

Applications

WB,IHC,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Asa, S. L., Ramyar, L., Murphy, P. R., Li, A. W., Ezzat, S. The endogenous fibroblast growth factor-2 antisense gene product regulates pituitary cell growth and hormone production. Molec. Endocr. 15: 589-599, 2001. 2. Gagnon, M. L., Moy, G. K., Klagsbrun, M. Characterization of the promoter for the human antisense fibroblast growth factor-2 gene; regulation by Ets in Jurkat T cells. J. Cell. Biochem. 72: 492-506, 1999. 3. Murphy, P. R., Knee, R. S. Identification and characterization of an antisense RNA transcript (gfg) from the human basic fibroblast growth factor gene. Molec. Endocr. 8: 852-859, 1994.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

72 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse<br> ELISA, 0.1-0.5 μg/ml, -<br>