Anti-SURF2 Antibody Picoband®, FITC Conjugate

Boster Bio Anti-SURF2 Antibody Picoband® catalog # A14641. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

SURF2 is a protein which in humans is encoded by the SURF2 gene. Surfeit 2, also known as SURF2, belongs to the SURF2 family and interacts with beta-1, 4-Gal-T3, uPAR and WDR20. SURF2 is located in the surfeit gene cluster, which is a group of very tightly linked genes that do not share sequence similarity. The SURF2 gene maps to human chromosome 9q34.2 and shares a biional promoter with SURF1, which is located on the opposite strand. The intergenic region between the SURF1 and SURF2 genes is expected to have biional promoter activity, as is found in mouse. Recombinant human SURF2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Synonyms

DAP3-binding cell death enhancer 1; Death ligand signal enhancer; DELE1; DELE; KIAA0141

Gene Name

DAP3 binding cell death enhancer 1

Gene ID

6835

UniProt

Q15527

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SURF2 recombinant protein (Position: D7-K230).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Essential for the induction of death receptor-mediated apoptosis through the regulation of caspase activation.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Duhig, T., Ruhrberg, C., Mor, O., Fried, M. The human surfeit locus. Genomics 52: 72-78, 1998. 2. Lennard, A., Gaston, K., Fried, M. The Surf-1 and Surf-2 genes and their essential biional promoter elements are conserved between mouse and human. DNA Cell Biol. 13: 1117-1126, 1994. 3. Yon, J., Jones, T., Garson, K., Sheer, D., Fried, M. The organization and conservation of the human Surfeit gene cluster and its localization telomeric to the c-abl and can proto-oncogenes at chromosome band 9q34.1. Hum. Molec. Genet. 2: 237-240, 1993.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64099 MW

Observed Molecular Weight

38 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -